Engagement · Integrated Human Practices · Overview

Integrated Human Practices

Six experts across three countries, what each of them told us, and the specific change every conversation produced.

In plain language

Our initial framework treated expressing an enzyme in a probiotic as a direct way to lower satiety signals and raise appetite. Eight people took that framework apart, each from a different direction, and what they left behind is a smaller and more defensible project.

Start with the overview

The narrative this record sits under, where the project started, what the six of them said, and the four things that changed, is on the Engagement overview.

The record

The hardest questions anyone asked us


This page explains how we recorded those conversations, who the end user actually is, where we stand with every stakeholder, and what changed as a result. The conversations themselves are the other three tabs above.

The record · 01

How we record an engagement


Early on we wrote meetings up as summaries. The summaries were useless, because they recorded that a conversation had been pleasant without recording what it did to the project. From the clinical webinars onward every engagement has been forced into four fields, and every record on this page uses them.

Four linked fields in sequence: Question, Engagement, Finding, and Consequence, with Consequence outlined in green.
Figure 1. The four-field record used for every engagement on this page.
The record · 02

Who the end user actually is


It is tempting to say our end user is a malnourished child. That is the beneficiary, not the user. A child does not decide to take a probiotic, does not obtain it, and does not administer it. If we design for the beneficiary and ignore the chain of people who have to agree before anything reaches them, we build something nobody can deliver.

Everyone in the chain between a procuring organization and a child
WhoTheir role in the chainWhat they need from us
The childBeneficiary. Severe acute malnutrition, failing or at risk of failing an appetite test.Something that works fast enough to matter, and does no harm while it does.
The caregiverDecides whether the child receives it at all, and administers each dose.A reason to trust a living organism given to an already sick child — and food worth being hungry for.
The frontline health workerRuns the appetite test, decides home treatment or admission, dispenses.A clear place in the existing protocol, and no new equipment.
The clinician or dietitianOwns the nutrition plan and carries the clinical risk.Evidence of safety, and an honest account of what the mechanism does not cover.
The procuring NGO or ministryBuys, stores and distributes at scale, inside a government-approved setting.Shelf stability, a cost per dose, and a regulatory status they can act on.
Five links drawn left to right in the direction of delivery, each marked with a filled, half-filled or hollow circle according to whether we reached that group.
Figure 2. The delivery chain, with our engagement marked against each link.
The record · 03

Everyone with a stake, and where we stand with them


Every stakeholder group, their stake, and our status with them
StakeholderStakeStatusDocumented in
Dr. Nalini RaghunathanMicrobiome science and live biotherapeutics; our chassis and assay designConsultedTechnical Expertise
Dr. Rajasree NambronEndocrinology; the appetite circuit our target sits insideConsulted, recordedClinical Expertise
Ms. Gabriela GardnerClinical dietetics; barriers to eating and probiotic safetyConsulted, recordedClinical Expertise
Dr. Mayra Corona MorenoBehavior analysis; why an individual child refuses a plateConsulted, recordedClinical Expertise
Dr. Rajashekar GFood availability, crop diversity and community trustConsulted, recordedPublic Outreach
Dr. Robert L. Bender IIGeriatric medicine; appetite loss and failure to thrive in older adultsConsulted, recordedClinical Expertise
Dr. Sara SchutteGeneral pediatrics; undernutrition and feeding in childrenConsulted, recordedClinical Expertise
Dr. Bharat PuchakayalaGastroenterology; the gut compartment our organism acts inConsulted, recordedClinical Expertise
Tamanna FerdousPublic health delivery in a high-burden settingCited as expert consultantPublic Outreach
Families at the expoCaregivers of children with sensory-driven food refusalEngaged in personPublic Outreach
Students and publicScientific literacy; future team membersOngoingEducation
Donors and sponsorsFund the work; require a plain-language accountOngoingEntrepreneurship
RegulatorsDecide whether a live engineered organism can be given to a childNot approached—
Frontline health workersWould run the appetite test and dispenseNot approached—
The record · 04

Which conversations we still owe


We assessed each stakeholder group on two axes: how much influence they have over whether this project could ever reach a child, and how much our engagement with them has actually changed it. The gap between the two is the agenda for next year.

A scatter of stakeholder groups, influence on the vertical axis against effect on the project on the horizontal, with a dashed box in the upper left holding three groups.
Figure 3. Stakeholder influence against effect on the project.
The record · 05

Conversation by conversation


This table is the test of whether any of the above counts as integrated. Every entry in the right-hand column points at something on another page of this wiki.

Every engagement, what we heard, and what we changed
InputWhat we heardWhat we changed
Dr. Corona Moreno 6 JulSeven causes of food refusal; appetite is one. The interventions that work are behavioral, not molecular.Reframed the probiotic as one part of a spectrum of care. Built the $3 Meal Plan and took a booth at the expo.
Tamanna Ferdous 15 JulComorbidities, hygiene, duration, cost, distribution. And: a hungrier child may just eat more cheap food.Stopped calling it a solution. Implementation questions now part of how we describe it. Committed to a screening-tool project.
Expo families 26 JulAffordable and sensory-acceptable are two different requirements.Sensory acceptability made a separate evaluation axis. Dropped the broad public description of the project.
Ms. Gardner 12 SepTwelve barriers to eating. Probiotics are withheld in ICU for translocation risk. BMI was never built to diagnose an individual.Added an exclusion criterion for active infection or barrier compromise. Repositioned to the recovery phase. Dropped BMI as an outcome measure.
Dr. Rajashekar G 20 SepLoss of crop diversity and food access drive malnutrition. Nutrient gardens raised vegetable consumption threefold.Project described as working alongside food-access approaches. Independence and locally built trust added as design principles.
Dr. Raghunathan 3 OctSpatial mismatch, host redundancy, no kinetic evidence for our enzyme choice. Commercial kits measure the wrong thing.Assay moved to LC-MS/MS OEA depletion, with BSA carrier, no-enzyme control and a catalytic-dead mutant as the load-bearing control.
Dr. Nambron 4 OctAppetite is a redundant, defended loop. Hedonic pathways override fullness. Gut hormones peak 15–20 minutes in.Narrowed the claim to lowering one satiety signal. Endpoint stays at OEA hydrolysis, not feeding behavior.
Dr. Schutte 16 OctIn children, eating is not always appetite. Refeeding after bowel rest is the rate-limiting step. Cyproheptadine already works, and sedates.Added hyperphagia syndromes to the exclusion criteria. Added a short-term inpatient indication. Named cyproheptadine as the comparator we have to beat.
Dr. Bender 17 OctOEA is not uniform across malnourished patients; the level moves with the underlying illness. Traditional appetite stimulants fail in older adults. Isolation outweighs much of the medicine.Baseline OEA stated as something to be characterized rather than assumed. Recorded advanced dementia with failure to thrive as an adjacent indication.
Dr. Puchakayala 18 OctOEA rises immediately on refeeding. The chassis and the mechanism read as novel to a gastroenterologist. Mouse models are the next step.Endpoint timing added to the Measurement question: when OEA is read, not only what. Nothing else, the rest of the session was an endorsement.

The striking thing about that table is how much of it agrees with itself. Five people who have never met, working in behavior analysis in Texas, clinical dietetics in Houston, general pediatrics in Iowa, public health in Bangladesh and sustainable agriculture in India, told us the same thing in five different vocabularies: appetite is one item on a list, and the items above it are usually more important.

We did not plan that convergence, and we could not have manufactured it. It is the reason we are confident the change it produced is real rather than politeness. A single expert telling a student team their premise is too narrow is an opinion. Five independent experts say it is a finding.

The project did not get bigger because of Human Practices. It got smaller, and true.

Two changes matter more than the rest, and both came from questions we did not think to ask. Ms. Gardner mentioned in passing that clinicians withhold probiotics in intensive care because of translocation risk, not knowing our chassis is a live organism destined for a gut that malnutrition has already made permeable. That single remark produced our exclusion criterion and moved the whole intervention from the acute phase to the recovery phase. Dr. Raghunathan pointed out that host enterocytes already express FAAH, which is the kind of fact that is obvious once said and invisible until somebody says it. Neither appeared on any list of questions we had prepared.

We would change one thing about how we did this. Our first expert conversation, with Dr. Corona Moreno on 6 July, shaped everything public we built. The other eight landed in September and October, after the meal plan and the expo were finished. That worked, but it worked on the strength of one well-chosen early conversation rather than by design. The recommendation we would pass to next year’s team is to front-load the expert sessions, write the Questions down before each one.